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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene has actually been observed in many tumors, which results in chromosome instability, and so may perhaps add to tumorigenesis. This protein was located to associate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a critical purpose in advertising and marketing cell-cycle development while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are already described. Two more splice variants have been claimed but specific nucleotide sequence details is not really nevertheless accessible.

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Blocking peptides are peptides that bind especially to your target antibody and block antibody binding. These peptide normally contains the epitope acknowledged because of the antibody.

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This guideline outlines even further achievable optimization of cellular and immunoassay parameters to ensure the very best benefits are...

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This manual outlines additional achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...

Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from your blocked antibody compared to the antibody alone, one can see which staining is specific.

Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of each and every mitotic function. Cyclin E forms a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary which is degraded as cells progress as a result of S period.

The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...

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